Chemiluminescent detection of DNA: application for DNA sequencing and hybridization
نویسندگان
چکیده
منابع مشابه
New algorithms for DNA sequencing by hybridization
The reconstruction of DNA sequences from DNA fragments is one of the most challenging problems in computational biology. In recent years the specific problem of DNA sequencing by hybridization has attracted quite a lot of interest in the optimization community. Despite the fact that well-working constructive heuristics are often the basis for well-working metaheuristics, only two constructive h...
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Sequencing by hybridization (SBH) is a promising alternative approach to DNA sequencing and mutation detection. Analysis of the resolving power of SBH involves rather difficult combinatorial and probabilistic problems, and sometimes computer simulation is the only way to estimate the parameters and limitations of SBH experiments. This paper describes a software package, DNA-SPECTRUM, which allo...
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Immobilization of single-stranded (ss) DNA on an electrode covered with an aluminum alkanebisphosphonate film by immersion in an ss-DNA solution was demonstrated with ss-DNA labeled with R~(bpy)3~+. This was detected by observing the electrogenerated chemiluminescence (ECL) produced upon oxidation in a solutior containing trin-propylamine. Surface hybridization was shown by immobilization of un...
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MOTIVATION It is widely recognized that the hybridization process is prone to errors and that the future of DNA sequencing by hybridization is predicated on the ability to successfully cope with such errors. However, the occurrence of hybridization errors results in the computational difficulty of the reconstruction of DNA sequencing by hybridization. The reconstruction problem of DNA sequencin...
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We consider the problem of sequence reconstruction in sequencing-by-hybridization in the presence of spectrum errors. As suggested by intuition, and reported in the literature, false-negatives (i.e., missing spectrum probes) are by far the leading cause of reconstruction failures. In a recent paper we have described an algorithm, called "threshold-theta", designed to recover from false negative...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.13.5115